Mass Spectrometry and Separations Facility
The Mass Spectrometry and Separations Facility (MS&SEP) provides advanced analytical support for research and industrial applications and supports the study of biological molecules of all sizes – from small metabolites to large macromolecular complexes.
Based across the Manchester Institute of Biotechnology (MIB) and the Department of Chemistry, we deliver high‑quality analysis, expert training and specialist support across a wide range of scientific disciplines.
Working with us
Led by Dr Kat Hollywood and a team of 12 technical specialists, the Mass Spectrometry and Separations Facility maintain a suite of world‑leading analytical instrumentation.
We specialise in solving complex analytical challenges and supporting researchers at every stage – from experimental design to data interpretation. We offer both service‑led analysis and collaborative project support. Users can also access training that enables them to operate instruments independently and collect their own data.
The facility offers:
Gas chromatography and GC-mass spectrometry
High-resolution liquid chromatography-mass spectrometry, including ion mobility
High-throughput UPLC screening
MALDI-MS & DESI-MS, including imaging and high-throughput analysis
QQQ-based quantitative assay development
Multi'omics – metabolomics, lipidomics, proteomics
HDX and intact protein analysis
Chiral screening
60 instruments
190+ active users
13 technical specialists
66,000 annual instrument hours
90+ active research projects
Our equipment
Separations
Range of HPLCs & UPLCs – varying detectors including UV, ELSD, RI, fluorescence and CAD with analytical, semi-preparative and preparative capabilities. We also offer SFC and ion chromatography alongside a chiral HPLC screening service.
Gas Chromatography/Gas Chromatography Mass Spectrometry Analysis
Options for liquid injection and headspace analysis, Vacuum ITEX/SPMe online sample concentration, true gas analysis (TCD/Methaniser), high throughput FID and accurate mass (GC-QToF) with a range of column chemistries. Novel capabilities in real-time monitoring of volatiles using proton transfer reaction mass spectrometry (PTR-MS).
Liquid Chromatography Mass Spectrometry
A variety of instrumentation is available with a range of mass accuracies, a range of ionisations and fragmentation technologies, ion mobility, untargeted vs. targeted and a vast range of column chemistries. Applications across metabolomics, lipidomics, and proteomics alongside accurate mass structure elucidation and confirmation and high-throughput.
Our impact
Our projects support the MIB’s research missions by accelerating technology development, understanding biomanufacturing routes to sustainable chemicals and materials, advancing platforms for advanced therapeutics, and providing biological solutions for environmental protection. Working with MIB’s cross-disciplinary teams, we provide collaborative access, training and clear handover so partners can adopt, sustain and grow capability.
